FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10585887
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1114331.0526562393874033No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCCTAAATCTCGTATGCCGTCTTCTGCTTG355130.3354749583100594TruSeq Adapter, Index 27 (97% over 39bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137880.13024888703232898No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112970.10671755706441982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG40700.051.38813445
TATGCCG41350.050.58033848
CGTATGC43450.048.1357246
CTCGTAT44500.046.7347744
GTATGCC48500.043.30611847
GCCTAAA50650.041.17664736
TGCCTAA51850.040.62191835
GCCGTCT53100.039.4434451
ATGCCTA54050.038.8593334
CCTAAAT54450.038.30297537
ATGCCGT55500.037.84408649
ATCTCGT57400.036.2830542
ACGTCTG59700.035.6758115
CTAAATC58700.035.42925338
ACACGTC60450.035.28198613
TGCCGTC59750.035.25098850
CGTCTGA61150.034.878116
CACGTCT64400.033.16375414
CACACGT67250.031.75830312
ACATGCC70400.030.08588432
Produced by FastQC (version 0.11.5)