FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13891258
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1498551.0787719873894792No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGGTCAATCTCGTATGCCGTCTTCTGCTTG340820.24534854942583315TruSeq Adapter, Index 25 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142690.10271927855634098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG45100.048.9911345
TATGCCG45350.048.52591348
CGTATGC48450.045.664646
CTCGTAT50050.043.91009544
GTATGCC56000.039.45535747
GCCGTCT62950.035.14627551
CGTCTGA65200.034.3404816
ACGTCTG65850.033.95670715
ATGCCGT65700.033.71993649
TCTCGTA67400.032.913243
ACACGTC69000.032.6630313
CACGTCT72650.030.940814
ATCTCGT73150.030.1647342
TGCCGTC74500.029.89529850
GTCAATC76850.028.98112738
CACACGT80000.028.13498912
GGTCAAT81550.027.34702137
TGGTCAA86900.025.96891836
AGTGGTC87700.025.86657534
GATCGGA88450.025.2688541
Produced by FastQC (version 0.11.5)