FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13933323
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1496371.073950557236059No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGGTCAATCTCGTATGCCGTCTTCTGCTTG342020.24546908156797914TruSeq Adapter, Index 25 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143240.10280390399332592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG44100.049.3001245
TATGCCG45600.047.41980748
CGTATGC46850.046.15476646
CTCGTAT49200.044.1297844
GTATGCC53850.040.2098647
GCCGTCT63250.034.51372551
CGTCTGA64800.033.5047416
ATGCCGT65300.033.24951649
TCTCGTA67300.032.8311343
ACGTCTG67450.032.3633315
ACACGTC69700.031.31871813
ATCTCGT71850.030.58782442
TGCCGTC72950.030.08626650
CACGTCT75850.029.01260614
CACACGT76350.028.78407712
GTCAATC76600.028.42055938
GGTCAAT82000.026.6928637
AGTGGTC88700.024.7097734
TGGTCAA89300.024.70891236
GATCGGA89650.024.4759451
Produced by FastQC (version 0.11.5)