FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13429964
Sequences flagged as poor quality0
Sequence length65
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA451660.33630767736979783No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACATTGGCATCTCGTATGCCGTCTTCTGCTTG233410.1737979342312459TruSeq Adapter, Index 5 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG34200.043.38723445
TATGCCG34350.043.1123748
CGTATGC35400.041.75028246
CTCGTAT39950.037.2160844
GTATGCC46100.032.37982647
CGTCTGA51600.030.07045216
TCTCGTA51900.029.0450443
ATGCCGT52200.028.42643449
ACGTCTG55250.028.1906815
GCCGTCT54500.027.60568851
ATCTCGT59400.025.17908742
ACACGTC63750.024.89483313
TGCCGTC61200.024.43888950
CACGTCT69450.022.59661114
CACACGT71450.022.37712112
GATCGGA72250.022.2780421
CATCTCG73600.020.56165141
ACATTGG76000.020.45506934
GTCACAC76400.020.11638329
TCGGAAG81550.019.786423
Produced by FastQC (version 0.11.5)