FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13399327
Sequences flagged as poor quality0
Sequence length65
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA446120.3329420947783422No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACATTGGCATCTCGTATGCCGTCTTCTGCTTG234130.1747326563490838TruSeq Adapter, Index 5 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG30350.045.48821645
TATGCCG31150.044.2252848
CGTATGC33450.041.3607646
CTCGTAT37700.036.54157644
GTATGCC44100.031.70673447
TCTCGTA47550.029.2201343
ATGCCGT48650.028.49881449
CGTCTGA51550.028.32619916
ACGTCTG53250.027.47728515
GCCGTCT51800.027.1074751
ACACGTC59100.024.90719413
ATCTCGT57050.024.3026742
CACGTCT60600.024.29068214
TGCCGTC59250.023.49986650
CACACGT67750.021.72716312
GTTACGA8000.020.64951311
GATCGGA71950.020.5597361
ACATTGG70250.020.24009134
GTCACAC72250.019.96651529
CATCTCG71250.019.58339741
Produced by FastQC (version 0.11.5)