FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18713687
Sequences flagged as poor quality0
Sequence length65
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1189260.6355027739856929No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCTCGTATGCCGTCTTCTGCTTG375890.20086367801278285TruSeq Adapter, Index 1 (97% over 38bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187920.10041847980037284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG50050.044.67616745
TATGCCG50350.044.40997748
CGTATGC53800.041.78144546
TATCTCG53600.041.16684341
CTCGTAT55000.040.44077344
GTATGCC62000.036.30309347
GCCGTCT72000.031.05622351
TCTCGTA72950.030.9752143
ACGTCTG78800.029.57418615
ACACGTC79450.029.4808313
GTATCTC77450.029.06122240
ATGCCGT79100.028.67877849
ATCTCGT79550.028.18280442
CGTCTGA84050.027.58651216
CACGTCT85950.027.3198314
CTGTATC86450.025.86514938
ACTGTAT89000.025.3230137
TGCCGTC90450.025.08005750
CACACGT94600.024.94676812
GTCACAC96350.023.88195629
Produced by FastQC (version 0.11.5)