FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14988210
Sequences flagged as poor quality0
Sequence length65
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1634191.0903169891534745No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTATGCCGTCTTCTGCTTG537150.35838168800677334TruSeq Adapter, Index 7 (97% over 39bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192950.12873451866500404No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152340.10163988895271685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG65900.048.0760345
TATGCCG66150.047.84974348
CGTATGC68000.046.63471246
CTCGTAT69300.045.54705444
GTATGCC75350.042.28148747
GCCGTCT78150.040.65349651
ATCTCGT87700.036.12554642
ACACGTC91900.035.69431313
ACGTCTG91900.035.46985215
GATCTCG91000.034.815541
CGTCTGA95500.034.00921216
ATGCCGT93850.033.8525349
CACGTCT98350.033.383414
ACCAGAT97200.032.83754732
CACACGT102200.032.29909512
TGCCGTC102350.031.01231450
TCTCGTA104400.030.48812343
GCACACG114800.028.8826511
CCGTCTT110800.028.75389152
GATCTGA111050.028.74209836
Produced by FastQC (version 0.11.5)