FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13216015
Sequences flagged as poor quality0
Sequence length65
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATCAAGTATCTCGTATGCCGTCTTCTGCTTG432150.32698964097725375TruSeq Adapter, Index 9 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA228850.1731611230768125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG53300.050.58756345
TATGCCG54250.049.75626448
CGTATGC56850.047.42915346
CTCGTAT57900.046.4664444
TATCTCG57950.046.2736341
GTATGCC63200.042.80392547
AAGTATC70800.038.04003538
CGTCTGA73300.037.1048216
GCCGTCT73250.037.0919851
ATGCCGT73250.036.93074449
CAAGTAT75550.036.03882237
GTATCTC77100.035.16193440
ACACGTC78500.034.9479413
TCAAGTA78250.034.7953136
ACGTCTG79350.034.57318515
AGTATCT78550.034.39954439
ATCAAGT80950.033.92627735
ATCTCGT79550.033.89449342
TGCCGTC85100.032.204250
CACGTCT86800.031.9796214
Produced by FastQC (version 0.11.5)