FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13179906
Sequences flagged as poor quality0
Sequence length65
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATCAAGTATCTCGTATGCCGTCTTCTGCTTG423890.3216183787653721TruSeq Adapter, Index 9 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA229690.17427286658948857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG51600.049.7943445
TATGCCG52950.048.524848
CGTATGC55400.046.4320946
TATCTCG55550.045.82877741
CTCGTAT56150.045.6542844
GTATGCC62900.040.9425747
GCCGTCT68700.037.7436351
AAGTATC70450.036.13624238
GTATCTC71850.035.7195240
ATGCCGT72800.035.53691549
CAAGTAT72950.035.4639837
CGTCTGA73500.035.35914216
TCAAGTA75550.034.51683436
ATCTCGT75500.034.14884642
AGTATCT76500.033.4326539
ACGTCTG78700.033.13528415
ATCAAGT78550.033.1234535
ACACGTC80250.032.5319213
TGCCGTC82000.031.69374550
CACGTCT84600.030.9289214
Produced by FastQC (version 0.11.5)