FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10164820
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA634800.6245068776426932No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTGATCATCTCGTATGCCGTCTTCTGCTTG482710.4748829787443358TruSeq Adapter, Index 2 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG56150.053.53704548
TCGTATG57800.052.0582445
CGTATGC58100.051.8417446
GTATGCC61700.048.912847
CTCGTAT62600.047.97230544
ACCGCTG66250.045.28321532
ATGCCGT66750.045.16786649
TCACCGC67050.044.69892530
GCCGTCT67350.044.63407551
CACCGCT68900.043.45592531
TGCCGTC71250.042.3151650
ATCTCGT71000.042.1307242
CATCTCG71900.041.56232541
ACGTCTG76400.039.34440615
CGTCTGA76800.039.177916
GATCATC76750.039.0883138
ACACGTC79850.037.9771613
CGCTGAT81350.036.9503734
GCTGATC81700.036.82818235
CACGTCT82500.036.5783314
Produced by FastQC (version 0.11.5)