FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10843580
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA766810.7071557548337357No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAAGCTAATCTCGTATGCCGTCTTCTGCTTG306870.2829969438137589TruSeq Adapter, Index 5 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG41050.050.80796448
TCGTATG41250.050.41882345
CGTATGC42800.048.66228546
CTCGTAT46350.044.8072744
GCTAATC46500.044.40732638
GTATGCC49750.042.0421347
CTAATCT50700.040.9031639
CAAGCTA52900.039.48036235
ATGCCGT54450.038.46679749
AAGCTAA56100.037.28094536
GCCGTCT56850.036.9468251
CGTCTGA57900.036.68237716
ACACGTC59250.035.5478513
ACGTCTG59800.035.2209115
AGCTAAT59150.035.20914537
ATCTCGT61100.033.9423342
TGCCGTC63100.033.3338750
TCTCGTA63800.032.59837743
CACGTCT65200.032.48481414
TAATCTC64850.032.20644840
Produced by FastQC (version 0.11.5)