FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10804211
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA768670.7114540802655558No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAAGCTAATCTCGTATGCCGTCTTCTGCTTG308630.28565713868416676TruSeq Adapter, Index 5 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG38000.048.05274248
TCGTATG38050.047.91206745
CGTATGC40050.045.7404246
CTCGTAT41250.044.33828444
GCTAATC42000.043.40605538
GTATGCC46850.039.1644447
CTAATCT47250.038.6455939
CAAGCTA49450.037.284235
ATGCCGT50200.036.4921149
CGTCTGA52550.035.30945616
GCCGTCT52150.035.0710351
ACGTCTG54550.034.1771215
AAGCTAA54800.033.53656836
AGCTAAT55500.033.27303737
ACACGTC56700.032.77695513
ATCTCGT56350.032.4570442
TGCCGTC57300.032.07336850
TAATCTC58600.031.2611640
TCTCGTA60000.031.0233943
CACGTCT62100.030.06929614
Produced by FastQC (version 0.11.5)