FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10131106
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1059911.0461937719336862No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTAGCCATCTCGTATGCCGTCTTCTGCTTG737770.7282225652362141TruSeq Adapter, Index 15 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130940.1292455137672037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG78850.053.3137345
TATGCCG78900.053.1303948
CGTATGC80000.052.47437746
CTCGTAT80250.052.19984444
GTATGCC83150.050.52194647
TCTCGTA88350.047.6812443
GCCGTCT88500.047.46685851
ATCTCGT91400.045.8316942
ACGTCTG92600.045.52223215
ACCTGTA94600.044.6221832
CATCTCG96100.043.55948641
ACACGTC97800.043.3431313
TAGCCAT98400.042.74907737
CACGTCT101100.042.1034314
CGTCTGA101150.041.79098516
ATGCCGT101300.041.5566449
CCTGTAG102150.041.2952433
CACACGT102950.041.1749212
AGCCATC107550.039.19441238
GTAGCCA107350.039.18499836
Produced by FastQC (version 0.11.5)