FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13109017
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA997030.7605680883623844No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAACCAATCTCGTATGCCGTCTTCTGCTTG352470.26887599581265326TruSeq Adapter, Index 1 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140310.10703319707343426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG45150.050.31009348
TCGTATG48200.047.2486245
CGTATGC48650.046.8119446
CTCGTAT49250.046.18121344
GTATGCC54650.041.56467447
ATGCCGT60200.037.9775949
GCCGTCT63400.036.15379751
TCTCGTA63050.036.12041543
CGTCTGA64550.035.73683516
ACCAATC66000.034.77332738
ACGTCTG68500.033.84835415
ATCTCGT67400.033.7454342
ACACGTC68900.033.6091613
AACAACC69600.033.14411534
TGCCGTC70100.032.78246350
AACCAAT73100.031.63789637
CACGTCT73450.031.5270614
TCACAAC76850.029.9790430
GTCACAA77650.029.82213829
CACACGT78700.029.72389412
Produced by FastQC (version 0.11.5)