FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13066278
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA998270.7640048681039849No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAACCAATCTCGTATGCCGTCTTCTGCTTG351380.26892126434168934TruSeq Adapter, Index 1 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142020.10869200854290717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG43350.048.04278648
TCGTATG44050.047.41327345
CGTATGC46950.044.54749346
CTCGTAT47600.043.877244
GTATGCC52600.039.8185347
ATGCCGT56750.036.80272749
TCTCGTA58800.035.77048543
GCCGTCT61250.034.29150451
CGTCTGA62450.033.96323416
ACCAATC61600.033.85722438
ACGTCTG63000.033.4794315
ATCTCGT65350.032.0046642
ACACGTC67000.031.8769213
AACAACC66450.031.6968334
TGCCGTC67850.031.17323950
AACCAAT70900.029.74900437
CACGTCT71450.029.68515214
TCACAAC72850.029.56100530
GTCACAA74800.028.67204929
CACACGT74950.028.45635612
Produced by FastQC (version 0.11.5)