FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11969883
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1062130.8873353231606358No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTATGCCGTCTTCTGCTTG336060.28075462391737666TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG39800.049.65948548
TCGTATG40800.048.65924545
CGTATGC41150.048.0303146
ACAACCG41600.047.43984232
CTCGTAT43200.045.75109544
CGAGAAT45550.043.06709337
AACCGAG47300.041.847934
ACCGAGA47400.041.57290635
CAACCGA48250.041.0849233
GTATGCC48650.040.8077547
ATGCCGT49450.040.02825549
GCCGTCT54150.036.8808351
CCGAGAA54100.036.42432436
TCTCGTA55650.035.83372543
CGTCTGA58100.034.06894316
TGCCGTC59700.033.40279850
ACACGTC60400.032.7714813
ACGTCTG60600.032.66345615
ATCTCGT61650.032.20271342
CACGTCT64000.031.2046414
Produced by FastQC (version 0.11.5)