FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8679191
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA843110.9714154233960284No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACGGAATCTCGTATGCCGTCTTCTGCTTG210050.24201564408480006TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG27000.047.52915248
TCGTATG27900.045.7839545
CGTATGC29100.043.9980946
CTCGTAT31500.040.64468444
GACGGAA32550.039.5125536
GTATGCC34100.037.71999747
CAAGACG35100.036.55814733
ATGCCGT35600.036.3788649
TCTCGTA37150.035.17806643
GCCGTCT37950.034.12615251
AGACGGA38050.034.0337235
AAGACGG38000.033.9234434
ACGGAAT40650.032.36524637
CGTCTGA41000.032.3044316
ACGTCTG40950.032.1277615
TGCCGTC43500.029.97557850
ATCTCGT43750.029.66891542
ACACGTC44750.029.66343913
ACAAGAC43650.029.46485332
GTCACAA46150.027.86870829
Produced by FastQC (version 0.11.5)