FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10616637
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA533940.5029276219955529No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGTACAATCTCGTATGCCGTCTTCTGCTTG374910.3531344247712341TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG45600.051.23557348
CGTATGC46950.049.76234446
TCGTATG47300.049.26939845
CTCGTAT47800.048.8157444
GTATGCC52950.044.29068847
TCTCGTA57050.041.15936743
GCCGTCT58000.040.53606851
AAGGTAC58300.039.92286734
GTACAAT58700.039.55031237
TACAATC59700.039.03588538
ATGCCGT62000.037.92083749
CGTCTGA63350.037.0661716
ACGTCTG63750.036.92614415
CAAGGTA63650.036.6599133
ATCTCGT64500.036.405342
CACGTCT67300.034.97833314
TGCCGTC68300.034.7685450
ACACGTC68050.034.54963713
GTCACAA69750.033.49689529
CACACGT74450.031.65886712
Produced by FastQC (version 0.11.5)