FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10848227
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA872480.8042604565704609No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACACAGAAATCTCGTATGCCGTCTTCTGCTTG253820.23397371754849897TruSeq Adapter, Index 23 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115380.10635839386473014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG31900.047.71758748
TCGTATG32450.046.908645
CGTATGC33600.045.21571746
CTCGTAT35750.042.4960644
GTATGCC41150.037.13482747
TCTCGTA43350.035.59066443
ATGCCGT44100.034.91816349
GCCGTCT44950.034.1264551
ACGTCTG46750.033.19002515
CGTCTGA46550.033.07914716
ACACGTC53050.029.3041213
TGCCGTC53400.029.05774750
ATCTCGT54850.028.02093342
CACGTCT57200.027.22960514
CACACGT59500.026.32576612
GAAATCT62400.024.72444539
AATCTCG61950.024.61901741
AAATCTC63600.024.58318940
GTCACAC64650.024.41117729
CAGTCAC69450.022.85143527
Produced by FastQC (version 0.11.5)