FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10802325
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA869050.8045027343650557No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACACAGAAATCTCGTATGCCGTCTTCTGCTTG249190.2306818208117234TruSeq Adapter, Index 23 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116450.10780086694299608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG32950.049.50882748
TCGTATG32800.049.3754945
CGTATGC35000.046.2718946
CTCGTAT36750.044.06846244
GTATGCC42850.037.93269347
TCTCGTA45200.036.15632243
GCCGTCT47900.034.48779751
CGTCTGA47950.034.4518316
ATGCCGT47550.034.36941549
ACGTCTG48850.033.8774915
ACACGTC53750.030.95377213
TGCCGTC55650.029.79093250
ATCTCGT57150.028.85416442
CACGTCT59400.028.158514
CACACGT60400.027.7899812
GTCACAC62850.026.61329829
GAAATCT61900.026.30639639
AATCTCG61900.026.1157741
AAATCTC63750.025.95945540
CAGTCAC69300.024.34914627
Produced by FastQC (version 0.11.5)