FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11735197
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1152820.9823610119199532No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTATGCCGTCTTCTGCTTG301890.25725175299571024TruSeq Adapter, Index 5 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135870.11577990552693747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG36550.049.63743248
TCGTATG38350.047.2303245
CTCGTAT39850.045.6005744
CGTATGC40850.044.5570646
GTATGCC47150.038.47838647
ATGCCGT50450.036.01978349
TCTCGTA52550.034.97287843
GCCGTCT52500.034.78186851
ACGTCTG57950.031.66212315
CGTCTGA58400.031.51917316
ATCTCGT58900.030.9019542
TGCCGTC60450.030.4027850
ACACGTC60650.030.25272613
CACGTCT64900.028.45330414
CACACGT66100.027.93687212
GTCACAC67450.027.07169529
GAATCTC76550.024.04638940
AGAATCT80500.023.34265939
GATCGGA80800.022.8074231
AGTCACA81750.022.66097828
Produced by FastQC (version 0.11.5)