FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13062488
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1624711.2437982718146803No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTCCAAATCTCGTATGCCGTCTTCTGCTTG330040.2526624330678811TruSeq Adapter, Index 8 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162180.12415705185719597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG44000.048.47279748
TCGTATG44850.047.4883645
CGTATGC47200.045.49899746
CTCGTAT49150.043.15367544
GTATGCC54150.039.71380247
ATGCCGT59150.036.30689249
GCCGTCT61300.035.2259851
CGTCTGA64400.033.6219316
TCTCGTA64500.033.2039243
ACGTCTG65150.033.0537615
ACACGTC68300.031.65889213
ATCTCGT68600.031.17642842
TGCCGTC71700.030.36334850
CACGTCT72650.029.92570114
CACACGT76050.028.93690312
GTCACAC81550.026.69663229
GATCGGA86450.024.9353851
AAATCTC86650.024.68208740
CAGTCAC93100.023.54307627
CAAATCT92300.023.26709239
Produced by FastQC (version 0.11.5)