FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11050967
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1279101.157455270656405No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGCTCGAATCTCGTATGCCGTCTTCTGCTTG321050.290517562852192TruSeq Adapter, Index 15 (97% over 36bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144360.1306311022374784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG41300.049.503948
TCGTATG41550.049.4132445
TCGAATC43850.046.0810338
CGTATGC44650.045.92186746
CTCGTAT44700.045.86469344
CGCTCGA46000.044.18375435
ACGCTCG46700.043.647834
GTATGCC50100.040.8674947
CTCGAAT51450.039.56078737
TCTCGTA54550.037.6370643
GCCGTCT54950.037.36781751
ACACGCT56200.036.58454532
CGTCTGA56500.036.54741716
ATGCCGT57150.035.92933749
CGAATCT57150.035.56347739
ACGTCTG58300.035.3178315
GCTCGAA58800.034.6156936
ATCTCGT61150.033.47836342
ACACGTC61450.033.45923613
TCACACG63400.032.4763630
Produced by FastQC (version 0.11.5)