FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12529538
Sequences flagged as poor quality0
Sequence length65
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA759020.6057845069786292No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGTATCAATCTCGTATGCCGTCTTCTGCTTG336490.2685573881495072TruSeq Adapter, Index 8 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC41950.049.71550434
TCGTATG42050.049.38701645
TATGCCG43250.048.0905148
CGTATCA43850.047.35953535
CACGTAT44850.046.8297633
CGTATGC44900.046.45467846
CTCGTAT45650.045.3628944
ACACGTA47200.044.435732
GTATGCC52650.039.5605847
GTATCAA55500.037.418336
GCCGTCT57200.036.67160851
ATGCCGT57600.036.31450749
CGTCTGA58550.036.1744516
TCTCGTA59150.035.20905343
ACGTCTG61900.034.26436215
TCACACG62250.033.7873830
TGCCGTC65950.031.98509450
ACACGTC68750.030.807613
CACGTCT73600.028.89760214
ATCTCGT74650.028.25404442
Produced by FastQC (version 0.11.5)