FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12541180
Sequences flagged as poor quality0
Sequence length65
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA769860.6138656809008403No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGTATCAATCTCGTATGCCGTCTTCTGCTTG340170.27124241897492896TruSeq Adapter, Index 8 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC42250.048.87248634
TCGTATG42750.048.50865645
TATGCCG42900.048.4860448
CGTATGC44250.047.07122846
CGTATCA44650.046.5097835
CACGTAT45350.045.72683733
ACACGTA48150.043.49658632
CTCGTAT47800.043.445544
GTATGCC53550.039.0070547
GCCGTCT56750.036.81017751
GTATCAA56750.036.48920436
ATGCCGT57550.036.09253749
CGTCTGA60750.034.42651416
TCTCGTA60900.034.2451243
ACGTCTG62300.033.52264815
TCACACG62450.033.30042630
TGCCGTC69050.030.46676650
ACACGTC69650.030.4509513
CACGTCT75100.028.04473714
TATCAAT75000.028.0034737
Produced by FastQC (version 0.11.5)