FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15070293
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA835610.5544749528094776No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTATGCAATCTCGTATGCCGTCTTCTGCTTG355370.23580828853161645TruSeq Adapter, Index 8 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG48800.048.78154845
TATGCCG48650.048.6970148
CGTATGC51650.046.0382146
CTCGTAT53750.043.95981644
GTATGCC60100.039.61490247
ACTATGC60300.039.3312534
CTATGCA64000.037.28786535
ATGCCGT64650.036.82806849
ACACTAT67700.035.38069532
TCTCGTA68250.034.92292443
GCCGTCT69200.034.6203251
CACACTA69700.034.57707631
CGTCTGA73300.033.20083216
CACTATG74100.032.5239233
ACGTCTG74750.032.39895215
ATCTCGT74850.031.84354842
AATCTCG74550.031.69449841
TGCCGTC77550.031.006850
ACACGTC78550.030.8692513
TATGCAA78250.030.49742336
Produced by FastQC (version 0.11.5)