FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10501434
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA478470.4556234891349124No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGCAATCTCGTATGCCGTCTTCTGCTTG272430.2594217132631601TruSeq Adapter, Index 14 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCAA34650.050.73948336
TCGTATG36500.048.7337245
TCGCAAT36200.048.40395737
TATGCCG36650.048.2175948
CGCAATC36900.047.40578538
CTCGTAT38750.045.67542344
GATCGCA41800.042.20150435
AGATCGC42150.042.20134
CGTATGC43850.040.70412446
TCTCGTA45650.039.09467743
GTATGCC47500.037.39000747
AATCTCG47700.037.04345341
GCCGTCT49200.036.15803551
ATCTCGT50050.035.42202842
ATGCCGT51350.034.70157249
CGTCTGA53100.033.66551616
ACGTCTG53700.033.12456515
CAGATCG55200.032.43789333
ACACGTC55350.032.08381313
CACGTCT59350.030.26939214
Produced by FastQC (version 0.11.5)