FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005740445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9270171
Sequences flagged as poor quality0
Sequence length65
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4819735.199181331175013No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTATGCCGTCTTCTGCTTG1170521.2626735795920054TruSeq Adapter, Index 5 (97% over 38bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223250.2408261940367659No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184090.1985831760816494No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169520.18286609815503943No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126470.13642682535198108No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102660.11074229375056836No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)