FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10514367
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA483230.4595901969181787No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGCAATCTCGTATGCCGTCTTCTGCTTG276040.26253601381804537TruSeq Adapter, Index 14 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG35700.049.50358248
ATCGCAA36300.048.4322936
TCGTATG36700.048.3882945
TCGCAAT38300.045.90318737
CGCAATC38600.045.39359338
CTCGTAT39450.044.7904644
GATCGCA42250.041.6814735
AGATCGC43550.040.70818334
CGTATGC44000.040.36541446
TCTCGTA46850.037.84151543
GTATGCC48650.036.50760747
AATCTCG49350.036.04355241
ATGCCGT49750.035.81986649
GCCGTCT51050.035.1393851
ATCTCGT52400.033.88962642
CGTCTGA53850.033.63396516
ACGTCTG54000.033.43128215
CAGATCG54500.032.5833233
ACACGTC55850.032.3238913
CACGTCT58750.030.72832914
Produced by FastQC (version 0.11.5)