FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12508029
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA788940.6307468586777342No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCAGGAAATCTCGTATGCCGTCTTCTGCTTG216800.17332866753027196TruSeq Adapter, Index 1 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG29500.046.5013648
TCGTATG30750.044.70449445
CTCGTAT35000.038.85436644
CGTATGC37150.036.92571346
TCTCGTA42750.032.017643
GTATGCC44400.031.29483647
ATGCCGT44900.030.68352749
CGTCTGA45950.030.17341616
GCCGTCT46400.029.94591951
ACGTCTG48050.028.97749315
ATCTCGT50200.027.50103842
ACACGTC51400.027.0887713
CACGTCT54700.025.4545314
AATCTCG55000.024.8864141
TGCCGTC58250.023.70198250
CACACGT60800.023.14329712
GGAAATC66950.020.84103438
GATCGGA67400.020.6796111
CCCCCCC241400.020.0983581
AAATCTC71450.019.44579340
Produced by FastQC (version 0.11.5)