FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16669279
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA927410.5563587963222644No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTATGCCGTCTTCTGCTTG450630.270335627593731TruSeq Adapter, Index 18 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG58900.049.53211245
TATGCCG59250.049.19445448
CGTATGC61600.047.31771546
CTCGTAT64500.045.0029844
GTATGCC72000.040.72878647
ATGCCGT75600.038.7893249
GCCGTCT77800.037.92008251
CGTCTGA84950.034.72543716
ACGTCTG87900.033.6271415
TGCCGTC92300.032.09082450
GTAATCT93350.031.41072539
ACACGTC94850.031.06976513
ATCTCGT94700.030.80719642
TAATCTC95750.030.65421540
TCTCGTA96150.030.61872543
CACGTCT99050.030.0799214
CACACGT104050.028.37930712
ATCCTGT117300.025.223734
GTCACAT119000.024.98798229
CAGTCAC123650.024.50157227
Produced by FastQC (version 0.11.5)