FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10417198
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA847780.8138272882976786No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGAGGAATCTCGTATGCCGTCTTCTGCTTG479960.460738098671063TruSeq Adapter, Index 25 (97% over 38bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119730.11493493739871316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG57650.051.16890745
TATGCCG58000.050.8645248
CGTATGC59900.049.2511246
CTCGTAT60850.048.3325944
GTATGCC66450.044.4408247
TCTCGTA69350.042.7489143
GCCGTCT70450.041.75006551
ATGCCGT73800.040.014849
CGTCTGA76100.039.2288416
ACGTCTG76950.038.79551715
ATCTCGT76650.038.60061642
ACACGTC78350.037.9516913
AATCTCG78550.037.441641
CACGTCT81100.036.84666414
TGCCGTC80550.036.77148450
AGGAATC83900.035.1244138
CATTGAG85050.034.6494733
CACACGT88100.033.81855812
ACATTGA89250.033.31637632
GAATCTC89300.033.23165540
Produced by FastQC (version 0.11.5)