FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12407991
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA612480.4936173793162809No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTAATCTCGTATGCCGTCTTCTGCTTG442990.3570199236927235TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG53700.049.5493145
TATGCCG54500.048.88497548
CGTATGC55550.047.7998846
CTCGTAT56750.046.5742344
ACTAATC61450.043.05914338
GACTAAT62550.042.63219537
GTATGCC63900.041.64623647
TCTCGTA66500.040.1000743
CTAATCT67000.039.4040939
AGACTAA68850.038.98827736
GCCGTCT69300.038.44566351
CGTCTGA72900.037.18614216
ATCTCGT72300.036.8830442
ACGTCTG73850.036.78766315
AAGACTA73750.036.51786435
ACACGTC76350.035.69899713
ATGCCGT75650.035.33485849
TAATCTC77100.034.5484240
CACGTCT80700.033.8843614
AATCTCG79100.033.52571541
Produced by FastQC (version 0.11.5)