FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13242495
Sequences flagged as poor quality0
Sequence length65
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA578110.43655670627023074No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCGTTAATCTCGTATGCCGTCTTCTGCTTG313530.23676051982651306TruSeq Adapter, Index 10 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAA39500.047.8703936
TCGTATG40000.047.27201545
TATGCCG40100.047.158448
CGTATGC42300.044.7057246
CTCGTAT45200.042.09453244
CGTTAAT45700.041.4404937
AGCGTTA48600.039.2711935
GTATGCC52450.036.1668747
GTTAATC53150.035.68730538
CAGCGTT54150.035.24616234
ATGCCGT54200.035.1624149
TCTCGTA57050.033.76462643
GCCGTCT57550.033.01307351
CGTCTGA59500.032.27540616
ACGTCTG64150.030.07383215
TGCCGTC66600.028.83714150
TAATCTC67450.028.20864140
ATCTCGT70900.026.96082542
ACACGTC73100.026.51260613
CCAGCGT74000.026.11052733
Produced by FastQC (version 0.11.5)