FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13252789
Sequences flagged as poor quality0
Sequence length65
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA587330.44317464044738053No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCGTTAATCTCGTATGCCGTCTTCTGCTTG318470.24030413522768682TruSeq Adapter, Index 10 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAA42100.048.0655436
TATGCCG42750.047.41445548
TCGTATG43700.046.37425645
CGTATGC45500.044.67641446
CTCGTAT47100.042.83843244
CGTTAAT48700.041.5515337
AGCGTTA49550.040.95779435
GTATGCC55150.036.8594747
ATGCCGT55950.036.3347749
CAGCGTT56800.036.19730434
GTTAATC56400.035.72180638
GCCGTCT59700.034.79430451
TCTCGTA60500.033.93534543
CGTCTGA63650.032.48713716
ACGTCTG66450.031.20700815
TGCCGTC68850.029.91309250
TAATCTC69150.029.39141740
ATCTCGT73450.027.63079642
ACACGTC75300.027.57842813
CCAGCGT75700.027.51063533
Produced by FastQC (version 0.11.5)