FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9667945
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA959040.9919791641346739No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATACCAAATCTCGTATGCCGTCTTCTGCTTG482870.49945464108453247TruSeq Adapter, Index 22 (97% over 38bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117390.12142187403838148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG58300.049.64425748
TCGTATG58400.049.5018245
CTCGTAT59800.047.9976144
CGTATGC60700.047.63007446
GTATGCC65150.044.51352347
TCTCGTA69300.041.4604243
ATGCCGT70450.041.25062649
GCCGTCT71500.040.76969551
ACGTCTG72000.040.47819515
CGTCTGA73100.039.86908716
CATACCA73950.039.17168434
CCATACC74450.038.82936533
ATCTCGT74800.038.37202542
ACACGTC77200.037.63705413
ATACCAA77300.037.5885635
AATCTCG76500.037.48036241
TGCCGTC79600.036.6210250
CACGTCT81600.035.78835314
CACACGT82500.035.25491312
CACCATA83550.035.02387231
Produced by FastQC (version 0.11.5)