FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10216673
Sequences flagged as poor quality0
Sequence length65
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1510051.4780251849109785No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTTCAATCTCGTATGCCGTCTTCTGCTTG440800.43145160856180875TruSeq Adapter, Index 5 (97% over 38bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114390.1119640415231064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG51200.052.4935948
TCGTATG52650.051.09868245
CGTATGC53500.050.2910246
CTCGTAT55100.048.5051344
GTATGCC59150.045.43734747
GCCGTCT64050.042.19363851
TCTCGTA64850.041.5307543
ATGCCGT65800.040.9363549
CGTCTGA69000.039.37394316
ACGTCTG70050.038.99430515
ATCTCGT69300.038.82096542
GTTCAAT71100.037.83778837
ACACGTC73250.037.41179313
CACGTCT74850.036.6513114
TGCCGTC74800.036.2480450
CACACGT79700.034.4581412
AATCTCG80250.033.41329641
TTCAATC82300.032.3659538
ACCCAGT85100.031.8903132
CAGTCAC90600.030.14986627
Produced by FastQC (version 0.11.5)