FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16249075
Sequences flagged as poor quality0
Sequence length65
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1173090.7219426336576082No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTGAGAATCTCGTATGCCGTCTTCTGCTTG458820.28236684242026083TruSeq Adapter, Index 16 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG57850.050.23192648
TCGTATG60350.048.24438545
CGTATGC60450.048.16901846
CTCGTAT67200.043.01920344
CGTGAGA67850.042.8679435
CCCGTGA74650.039.12108633
ATGCCGT76150.038.27665749
TCTCGTA77300.037.5891343
GTATGCC79600.036.65474347
GCCGTCT80300.036.37195251
CCGTGAG80950.036.14933834
CGTCTGA83300.035.44856316
ACCCGTG84000.034.66118632
ACGTCTG87250.034.080415
CACCCGT88650.032.8763631
TGCCGTC95750.030.56468250
ATCTCGT101250.028.69767642
GAATCTC102800.028.43715340
CACGTCT108750.027.50541914
AATCTCG105700.027.40577341
Produced by FastQC (version 0.11.5)