FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16256579
Sequences flagged as poor quality0
Sequence length65
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1178880.7251710215291913No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTGAGAATCTCGTATGCCGTCTTCTGCTTG463260.28496770445983743TruSeq Adapter, Index 16 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG57500.048.69353548
TCGTATG59200.047.33736445
CGTATGC60250.046.5186946
CGTGAGA65400.042.6239335
CTCGTAT66100.042.3959444
CCCGTGA73850.037.786833
TCTCGTA76950.036.80143443
GTATGCC76850.036.62408447
ATGCCGT77100.036.43002749
CCGTGAG79600.035.20545634
ACCCGTG79600.034.98310532
GCCGTCT81700.034.5236651
CGTCTGA83150.034.05705616
CACCCGT84350.033.08304631
ACGTCTG87850.032.1678415
TGCCGTC94000.030.19452350
ATCTCGT98000.028.77614642
TCACCCG100200.027.9381730
GAATCTC103400.027.24471540
ACACGTC105450.026.93877613
Produced by FastQC (version 0.11.5)