FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11836771
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1137720.9611742932257454No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCCTGAATCTCGTATGCCGTCTTCTGCTTG472920.3995346365997957TruSeq Adapter, Index 23 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127550.10775742810264724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG59050.052.2611348
TCGTATG59700.051.78351645
CGTATGC61100.050.6510546
CTCGTAT63250.048.45734444
GTATGCC68950.044.84217547
TCTCGTA70950.043.48926543
GCCGTCT74050.041.95476551
ATGCCGT75750.040.817649
CGTCTGA78000.039.9741716
ACGTCTG78800.039.60577815
TGCCGTC82950.037.4532950
ACACGTC84800.037.01219613
CACGTCT86550.036.36607714
ACCCTCC85950.036.0364932
ATCTCGT86250.035.87709842
CACACGT90600.034.77300312
GAATCTC89350.034.33492340
CCTGAAT92550.033.6578737
TGAATCT97400.031.7091839
CTGAATC98700.031.38132338
Produced by FastQC (version 0.11.5)