FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17743596
Sequences flagged as poor quality0
Sequence length65
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA762290.4296141548759338No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTCTGCTTG361630.20380874316570327TruSeq Adapter, Index 22 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG45000.047.8577748
TCGTATG45900.046.8511545
CGTATGC47700.045.2106946
ACCGAAC48700.044.27859532
CTCGTAT53450.040.2884544
CACCGAA54750.039.38570831
CCGAACT55400.039.1898433
TCACCGA55750.038.9967130
GTATGCC60150.036.1471447
ATGCCGT64900.033.63791749
CGAACTT66150.033.04408634
GTCACCG66100.032.9356629
GAACTTA66900.032.7177335
CGTCTGA67850.032.30365816
TCTCGTA67950.032.16874743
GCCGTCT67800.032.06859251
ACGTCTG72050.030.46152915
TGCCGTC79700.027.68760550
ACACGTC79650.027.36932413
CACGTCT83450.026.29977414
Produced by FastQC (version 0.11.5)