FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23705570
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG39250.024.07768243
CGTATGC40750.022.80486544
CTCGTAT41700.022.33919742
GATCGGA43200.020.4271351
AATCTCG47200.019.9714839
TCTCGTA47000.019.91555241
ACGTCTG49900.019.43147515
ACACGTC50050.019.2383913
ATCTCGT49200.019.02457840
ATCGGAA49400.017.7227572
CACGTCT55050.017.61363614
GTATGCC59750.016.00501645
CGTATCG15200.015.9899673
CACACGT61750.015.88468512
TCGGAAG60850.014.5714593
GCACACG70600.014.37146111
CCCGTAT21400.013.7102081
GTATCGA19300.013.40934
CGGAAGA72950.013.388444
GACCAAT73450.013.17066135
Produced by FastQC (version 0.11.5)