FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14391241
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG16700.025.86897943
CGTATGC18800.022.97935944
CTCGTAT19250.022.90972542
GATCGGA20250.020.8448451
AATCTCG23000.019.07442739
ACACGTC24100.018.6699913
TCTCGTA24550.018.23860441
ATCTCGT25650.017.36829840
ATCGGAA24000.017.2541942
ACGTCTG27500.016.4435115
CACGTCT30200.015.04788914
CGTCTGA33000.014.180140516
TCGGAAG32650.013.3712933
GTATGCC37100.012.00841945
CGACCTA5050.011.5860884
CACACGT38900.011.45108812
GTCACAC43700.011.01696529
CGGAAGA45050.010.6399534
GCACACG46050.010.35708111
TTGAATC45850.010.35315536
Produced by FastQC (version 0.11.5)