FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21808831
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG20300.020.61650843
CGTATGC23400.017.98224444
CTCGTAT25250.016.57443842
ACACGTC28900.014.94692413
TATCTCG30150.014.40235139
TCTCGTA29450.014.21042141
GATCGGA23750.014.1536221
ATCTCGT29600.014.06213840
ACGTCTG31700.013.62669115
CGCGATT3405.456968E-1212.6072271
CGTATCG11200.012.4544093
CACGTCT36150.012.13597814
CACTAGC36100.011.716639531
CGCGGAT9200.011.5253721
GTATCGA14150.011.4478654
ACTAGCT39850.011.06563132
ATCGGAA30750.010.7552782
CGCGAAT6850.010.5391191
GTCACTA41750.010.238811529
GCGCGAT13150.09.9505521
Produced by FastQC (version 0.11.5)