FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15103773
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG10850.018.4580443
CGTATGC12200.016.41609644
CTCGTAT13200.016.02361942
AGTCACG15150.013.95936928
CCCGTAT12150.013.9222031
GTATCGA12400.013.7893094
CGTATCG15650.012.5070263
CGCGTAT2251.0120457E-612.0287841
ACACGTC17650.011.85461213
CGCGTAA10500.011.5991841
CGCGATT2351.7097082E-611.516921
TCTCGTA19000.011.01322341
ATCGAAG26950.010.8526336
CCGTATC19650.010.7625312
AATCGGG16500.010.3628735
GTCACGG20450.010.34153729
GATCGGA14100.010.2372631
CACGTCT22000.010.12422214
TCGAAGG30800.010.0804277
CGACCTA4702.1827873E-1110.0524514
Produced by FastQC (version 0.11.5)