FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17502011
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG15800.027.05977645
CTCGTAT20250.021.3355544
CGTATCT21950.019.68027339
TATCTCG23600.018.30547341
GTTCCGT24800.017.23644635
TCCGTAT26450.016.50181837
TCTCGTA30650.015.6373543
ACACGTC30900.014.05220913
GATCGGA24250.013.6761151
TTCCGTA31450.013.66339136
CCGTATC36450.012.5300938
GTCGCTA16100.012.43694327
CACGTCT36300.012.33366914
ATCTCGT36450.011.91427542
AGTTCCG39150.011.89554534
GTATCTC39400.011.53528240
CCCGTAT11000.011.4855681
CCGGAAT23100.011.426971
CCGACAT24400.011.37293151
TATATCG11950.011.29611635
Produced by FastQC (version 0.11.5)