FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14297813
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG11950.028.05708945
CTCGTAT13700.024.80165544
TCTCGTA16600.020.87469743
ACCCGTC19000.018.94587732
GATCGGA15450.018.54051
ATCTCGT19700.017.70375642
CCCGATC20750.017.34845538
CCGATCT21350.017.0722739
ACACGTC21500.016.74286713
TCCCGAT21550.016.59996837
ACGTCTG23500.016.0838415
CGTCCCG23000.015.6509435
GTCCCGA24800.015.24073536
CACGTCT24950.014.87858714
CACCCGT27250.013.70553431
TCACCCG30400.012.43340530
CACACGT29950.012.16932512
ATCGGAA23550.012.1328022
CGTCTGA34350.011.26548816
GCGATCG3002.2357199E-811.2491143
Produced by FastQC (version 0.11.5)