FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21295605
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG24300.030.09546545
CTCGTAT30750.023.78275744
AGTCACG35200.020.900428
TCTCGTA36050.020.41028843
TGAAACG37850.019.85318235
ACACGTC39350.019.15356413
GATCGGA32100.018.2012461
GTCACGT40650.017.82152429
GAAACGA42450.017.80783536
AAACGAT42250.017.7858437
ACGATCT41700.017.7509539
ATCTCGT41200.017.7494442
ACGTCTG43050.017.5073815
AACGATC45600.016.52854538
CGTGAAA46700.016.09085533
TCACGTG47000.015.94027830
CACGTCT48300.015.60440414
CACGTGA48900.015.4129431
ATCGGAA39150.014.8839272
CACACGT51700.014.66523112
Produced by FastQC (version 0.11.5)