FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18868031
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG259080.13731162515049927TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG39150.035.40022345
CTCGTAT42850.032.5010144
TATCTCG49400.028.73758341
TCTCGTA48950.028.45083243
GATCGGA48000.028.22231
AGTCACG51400.027.53184728
GTCACGT51850.027.20612129
ATCTCGT52400.026.79175442
ACACGTC52650.026.66453713
ATCGGAA53900.025.1696052
ACGTCTG56750.024.817415
TCACGTG58400.024.3473730
CACGTCT60350.023.11331614
CACACGT62100.022.75180412
TCGGAAG60550.022.4796263
ACGTGGC63500.022.25019332
CGTCTGA66300.021.37838216
GCCTTAT66450.021.2624137
GGCCTTA68200.020.98072836
GCACACG72300.019.9465411
Produced by FastQC (version 0.11.5)