FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005740501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005740501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15138592
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG18350.029.18045645
CTCGTAT19850.026.63535744
GATCGGA22350.023.5404421
TATCTCG22950.022.84141241
TCTCGTA25150.021.02233943
ATCGGAA26600.019.622452
ATCTCGT28100.018.81530642
ACACGTC29800.018.11931813
ACGTCTG30350.017.7909615
CACGTCT34750.015.60303514
TCGGAAG33900.015.5960643
CACACGT37400.014.617784512
GCACACG43250.013.1087511
CGGAAGA42450.013.0907984
GTATCGA9850.012.7908274
CGTATCG9400.012.44578553
CGTCTGA44250.012.4057616
TTATCTC45000.012.24899440
CCCGTAT10700.011.606871
GTCACAT47850.011.47241229
Produced by FastQC (version 0.11.5)